Mouse AMCase/CHIA Protein, Ultra Low Endotoxin (AMC-MM101-UL)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Tyr22-Pro473
Catalog #AMC-MM101-UL
Regular price $290.00
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Mouse AMCase/CHIA Protein, Ultra Low Endotoxin (AMC-MM101-UL) Summary

Product Description

Recombinant Mouse AMCase/CHIA Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr22-Pro473.

Product Background

Acidic mammalian chitinase (AMCase) and chitotriosidase (CHIT-1) are two active chitinases expressed in humans. The chitinase activity of AMCase was found to be causative in allergic inflammation and its expression was found to be induced by interleukin-13. CHIT1-1 is expressed by phagocytic cells and extremely high levels are seen in lysosomal storage diseases. The expression of AMCase and CHIT-1 were differently modulated in HMMs at different stage of maturation.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Mouse AMCase/CHIA Protein, Ultra Low Endotoxin (AMC-MM101-UL) Specifications/Details

Protein
AMCase/CHIA
Synonyms
Acidic mammalian chitinase; AMCase; CHIA; CHIT2; EC 3.2.1.14; Chia1; YNL
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Tyr22-Pro473
Molecular Weight
The protein has a predicted MW of 51 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result.

Product Tag

C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.01 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Mouse AMCase on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse AMCase on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse AMCase is greater than 95% as determined by SEC-HPLC. The purity of Mouse AMCase is greater than 95% as determined by SEC-HPLC.
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