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Mouse AMCase/CHIA Protein, Ultra Low Endotoxin (AMC-MM101-UL) Summary
Product Description |
Recombinant Mouse AMCase/CHIA Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr22-Pro473. |
Product Background |
Acidic mammalian chitinase (AMCase) and chitotriosidase (CHIT-1) are two active chitinases expressed in humans. The chitinase activity of AMCase was found to be causative in allergic inflammation and its expression was found to be induced by interleukin-13. CHIT1-1 is expressed by phagocytic cells and extremely high levels are seen in lysosomal storage diseases. The expression of AMCase and CHIT-1 were differently modulated in HMMs at different stage of maturation. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse AMCase/CHIA Protein, Ultra Low Endotoxin (AMC-MM101-UL) Specifications/Details
Protein |
AMCase/CHIA |
Synonyms |
Acidic mammalian chitinase; AMCase; CHIA; CHIT2; EC 3.2.1.14; Chia1; YNL |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Tyr22-Pro473 |
Molecular Weight |
The protein has a predicted MW of 51 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Mouse AMCase on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Mouse AMCase is greater than 95% as determined by SEC-HPLC. |
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