Mouse AMCase/CHIA Protein, Ultra Low Endotoxin (AMC-MM101-UL)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Tyr22-Pro473
Catalog #AMC-MM101-UL
Regular price $290.00
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Mouse AMCase/CHIA Protein, Ultra Low Endotoxin (AMC-MM101-UL) Summary

Product Description

Recombinant Mouse AMCase/CHIA Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr22-Pro473.

Product Background

Acidic mammalian chitinase (AMCase) and chitotriosidase (CHIT-1) are two active chitinases expressed in humans. The chitinase activity of AMCase was found to be causative in allergic inflammation and its expression was found to be induced by interleukin-13. CHIT1-1 is expressed by phagocytic cells and extremely high levels are seen in lysosomal storage diseases. The expression of AMCase and CHIT-1 were differently modulated in HMMs at different stage of maturation.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Mouse AMCase/CHIA Protein, Ultra Low Endotoxin (AMC-MM101-UL) Specifications/Details

Protein
AMCase/CHIA
Synonyms
Acidic mammalian chitinase; AMCase; CHIA; CHIT2; EC 3.2.1.14; Chia1; YNL
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Tyr22-Pro473
Molecular Weight
The protein has a predicted MW of 51 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result.
Product Tag
C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse AMCase on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse AMCase on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse AMCase is greater than 95% as determined by SEC-HPLC. The purity of Mouse AMCase is greater than 95% as determined by SEC-HPLC.
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