Name: Human/Mouse/Rat BDNF-Protein (BDF-HE001)
Brand: KactusBio
SKU: BDF-HE001-100UG
Price: 330.0 USD
Availability: InStock

Human/Mouse/Rat BDNF-Protein (BDF-HE001)

Immobilized Human/Mouse/Rat BDNF, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.39 ug/ml determined by ELISA (QC Test).

Human/Mouse/Rat BDNF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human/Mouse/Rat BDNF, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.39 ug/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: His129-Arg247(Human) / His131-Arg249(Mouse/Rat)

Catalog #BDF-HE001

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human/Mouse/Rat BDNF-Protein (BDF-HE001) Summary

Product Description	Recombinant Human/Mouse/Rat BDNF-Protein is expressed from E.coli without tag. It contains His129-Arg247(Human) / His131-Arg249(Mouse/Rat).
Product Background	The brain-derived neurotrophic factor (BDNF) is a secretory growth factor that promotes neuronal proliferation and survival, synaptic plasticity and long-term potentiation in the central nervous system. Brain-derived neurotrophic factor biosynthesis and secretion are chrono-topically regulated processes at the cellular level, accounting for specific localizations and functions.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human/Mouse/Rat BDNF-Protein (BDF-HE001) Specifications/Details

Protein	BDNF
Synonyms	Abrineurin; BDNF; ANON2; BULN2
Accession	P23560-1(Human) / P21237-1(Mouse) / P23363(Rat)
Species	Human/Mouse/Rat
Biotinylated	no
Amino Acid Range	His129-Arg247(Human) / His131-Arg249(Mouse/Rat)
Molecular Weight	The protein has a predicted MW of 13.51 kDa. The protein migrates to 15-19 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human/Mouse/Rat BDNF, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.39 ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	No Tag
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human/Mouse/Rat BDNF, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.39 ug/ml determined by ELISA (QC Test).
	Human/Mouse/Rat BDNF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.