Human/Mouse/Rat BDNF-Protein (BDF-HE001) Summary
Product Description |
Recombinant Human/Mouse/Rat BDNF-Protein is expressed from E.coli without tag. It contains His129-Arg247(Human) / His131-Arg249(Mouse/Rat). |
Product Background |
The brain-derived neurotrophic factor (BDNF) is a secretory growth factor that promotes neuronal proliferation and survival, synaptic plasticity and long-term potentiation in the central nervous system. Brain-derived neurotrophic factor biosynthesis and secretion are chrono-topically regulated processes at the cellular level, accounting for specific localizations and functions. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human/Mouse/Rat BDNF-Protein (BDF-HE001) Specifications/Details
Protein |
BDNF |
Synonyms |
Abrineurin; BDNF; ANON2; BULN2 |
Accession |
|
Species |
Human/Mouse/Rat |
Biotinylated |
no |
Amino Acid Range |
His129-Arg247(Human) / His131-Arg249(Mouse/Rat) |
Molecular Weight |
The protein has a predicted MW of 13.51 kDa. The protein migrates to 15-19 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human/Mouse/Rat BDNF, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.39 ug/ml determined by ELISA (QC Test). |
Product Tag |
No Tag |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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