• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human B7-H7, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD28H, hFc Tag (Cat. CD8-HM28H) with the EC50 of 4.9ng/ml determined by ELISA.
  • Amino Acid Range: Ile23-Asn344
Catalog #BH7-HM477B
Price
Regular price $577.50
Regular price Sale price $577.50
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human B7-H7/HHLA2 Protein (BH7-HM477B) Summary

Product Description

 Recombinant Biotinylated Human B7-H7/HHLA2 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ile23-Asn344.

Product Background

 B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins.Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.

Biotinylated Human B7-H7/HHLA2 Protein (BH7-HM477B) Specifications/Details

Protein
 B7-H7/HHLA2
Synonyms
 B7H7; B7-H7; HHLA2; B7 Homolog 7
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Ile23-Asn344
Molecular Weight
 The protein has a predicted MW of 39.88 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human B7-H7, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD28H, hFc Tag (Cat. CD8-HM28H) with the EC50 of 4.9ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human B7-H7, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD28H, hFc Tag (Cat. CD8-HM28H) with the EC50 of 4.9ng/ml determined by ELISA. Immobilized Biotinylated Human B7-H7, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD28H, hFc Tag (Cat. CD8-HM28H) with the EC50 of 4.9ng/ml determined by ELISA.
Biotinylated Human B7-H7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human B7-H7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Biotinylated Human B7-H7, His-Avi Tag on Streptavidin-Biosensor can bind Human CD28H, hFc Tag (Cat. CD8-HM28H) with an affinity constant of 5.28 nM as determined in BLI assay . Loaded Biotinylated Human B7-H7, His-Avi Tag on Streptavidin-Biosensor can bind Human CD28H, hFc Tag (Cat. CD8-HM28H) with an affinity constant of 5.28 nM as determined in BLI assay .
The purity of Biotinylated Human B7-H7 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human B7-H7 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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