Human CD83 Protein (CD8-HM183) Summary
Product Description |
Recombinant Human CD83 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr20-Ala143. |
Product Background |
CD83 is a member of the immunoglobulin (Ig) superfamily and is expressed in membrane bound or soluble forms. Membrane CD83 (mCD83) can be detected on a variety of activated immune cells, although it is most highly and stably expressed by mature dendritic cells (DC). While CD83 is emerging as a promising immune modulator with therapeutic potential, some important aspects such as its ligand/s, intracellular signaling pathways and modulators of its expression are unclear. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human CD83 Protein (CD8-HM183) Specifications/Details
Protein |
CD83 |
Synonyms |
CD83; HB15; BL11; CD83 molecule |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Thr20-Ala143 |
Molecular Weight |
The protein has a predicted MW of 15.2 kDa. Due to glycosylation, the protein migrates to 18 kDa and 25-28 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD83, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD83 Antibody, hFc Tag with the EC50 of 29.2ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed