Human DLK1 Protein (DLK-HM201)
Human DLK1 Protein (DLK-HM201)
Human DLK1 Protein (DLK-HM201)
  • Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human DLK1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-DLK1 Antibody, hFc Avi Tag with the EC50 of 39.2ng/ml determined by ELISA.
  • Amino Acid Range: Ala24-Gln303
Catalog #DLK-HM201
Regular price $297.00
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Size
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Human DLK1 Protein (DLK-HM201) Summary

Product Description

Recombinant Human DLK1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ala24-Gln303.

Product Background

paternally inherited genetic defects of DLK1 were identified in four families with nonsyndromic CPP and a metabolic phenotype. DLK1 encodes a transmembrane protein that is important for adipose tissue homeostasis and neurogenesis and is located in the imprinted chromosome 14q32 region associated with Temple syndrome.

Product Category

Recombinant Protein / Antibody discovery / ADC targets

Human DLK1 Protein (DLK-HM201) Specifications/Details

Protein
DLK1
Synonyms
pG2; FA1; DLK; DLK1; DLK-1; Pref1; secredeltin; ZOG
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ala24-Gln303
Molecular Weight
The protein has a predicted MW of 56.58 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human DLK1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-DLK1 Antibody, hFc Avi Tag with the EC50 of 39.2ng/ml determined by ELISA.
Product Tag
C-hFc (IgG1)

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human DLK1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-DLK1 Antibody, hFc Avi Tag with the EC50 of 39.2ng/ml determined by ELISA. Immobilized Human DLK1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-DLK1 Antibody, hFc Avi Tag with the EC50 of 39.2ng/ml determined by ELISA.
Human DLK1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human DLK1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human DLK1 is greater than 95% as determined by SEC-HPLC. The purity of Human DLK1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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