Name: Cynomolgus/Rhesus macaque TNFSF15 Protein (active trimer) (FSF-CM115)
Brand: KactusBio
SKU: FSF-CM115-100UG
Price: 346.5 USD
Availability: InStock

Cynomolgus/Rhesus macaque TNFSF15 Protein (active trimer) (FSF-CM115)

Mouse DR3, His Tag immobilized on CM5 Chip can bind Cynomolgus/Rhesus macaque TNFSF15, His Tag with an affinity constant of 11.70 nM as determined in SPR assay (Biacore T200).

Immobilized Cynomolgus/Rhesus macaque TNFSF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test).

Immobilized Cynomolgus/Rhesus macaque TNFSF15, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human DcR3, hFc Tag with the EC50 of 20.8ng/ml determined by ELISA.

Cynomolgus/Rhesus macaque TNFSF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Cynomolgus/Rhesus macaque TNFSF15 is greater than 85% and the molecular weight of this protein is around 60-98 kDa as determined by SEC-MALS.

Loaded Human DR3, hFc Tag on ProA-Biosensor, can bind Cynomolgus/Rhesus macaque TNFSF15, His Tag with an affinity constant of 1.30 nM as determined in BLI assay (Gator).

The purity of Cynomolgus/Rhesus macaque TNFSF15 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus/Rhesus macaque TNFSF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). The affinity constant of 11.70 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Leu72-Leu251

Catalog #FSF-CM115

Size

100UG

500UG

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Cynomolgus/Rhesus macaque TNFSF15 Protein (active trimer) (FSF-CM115) Summary

Product Description	Recombinant Cynomolgus/Rhesus macaque TNFSF15 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Leu72-Leu251.
Product Background	TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Cynomolgus/Rhesus macaque TNFSF15 Protein (active trimer) (FSF-CM115) Specifications/Details

Protein	TNFSF15
Synonyms	TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A; TNF superfamily member 15
Accession	A0A2K5UA22(Cynomolgus) / F6S8F9(Rhesus macaque)
Species	Cynomolgus/Rhesus macaque
Biotinylated	no
Amino Acid Range	Leu72-Leu251
Molecular Weight	The protein has a predicted MW of 21.52 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Cynomolgus/Rhesus macaque TNFSF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). The affinity constant of 11.70 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Mouse DR3, His Tag immobilized on CM5 Chip can bind Cynomolgus/Rhesus macaque TNFSF15, His Tag with an affinity constant of 11.70 nM as determined in SPR assay (Biacore T200).
	Immobilized Cynomolgus/Rhesus macaque TNFSF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test).
	Immobilized Cynomolgus/Rhesus macaque TNFSF15, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human DcR3, hFc Tag with the EC50 of 20.8ng/ml determined by ELISA.
	Cynomolgus/Rhesus macaque TNFSF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Cynomolgus/Rhesus macaque TNFSF15 is greater than 85% and the molecular weight of this protein is around 60-98 kDa as determined by SEC-MALS.
	Loaded Human DR3, hFc Tag on ProA-Biosensor, can bind Cynomolgus/Rhesus macaque TNFSF15, His Tag with an affinity constant of 1.30 nM as determined in BLI assay (Gator).
	The purity of Cynomolgus/Rhesus macaque TNFSF15 is greater than 95% as determined by SEC-HPLC.