Name: Human FSTL3 Protein (FTS-HM1L3)
Brand: KactusBio
SKU: FTS-HM1L3-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human Activin A, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human FSTL3, His Tag with the EC50 of 7.6ng/ml determined by ELISA.
Amino Acid Range: Met27-Val263

Catalog #FTS-HM1L3

Size

100UG

500UG

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Human FSTL3 Protein (FTS-HM1L3) Summary

Product Description	Recombinant Human FSTL3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met27-Val263.
Product Background	Follistatin-like 3 (FSTL3) is a novel cytokine that regulates insulin sensitivity and counteracts activin/myostatin signalling. In the present study, regulation of FSTL3 in renal dysfunction was investigated in both human chronic kidney disease (CKD) and acute kidney dysfunction (AKD). Furthermore, mFSTL3 expression was analysed in insulin-sensitive tissues in a mouse model of CKD.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human FSTL3 Protein (FTS-HM1L3) Specifications/Details

Protein	FSTL3
Synonyms	FLRG; FLRGFSRP; Follistatin-like 3; FSTL3
Accession	O95633-1
Species	Human
Biotinylated	no
Amino Acid Range	Met27-Val263
Molecular Weight	The protein has a predicted MW of 26.1 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Activin A, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human FSTL3, His Tag with the EC50 of 7.6ng/ml determined by ELISA.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human Activin A, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human FSTL3, His Tag with the EC50 of 7.6ng/ml determined by ELISA.
	Immobilized Human FSTL3, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FSTL3 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA.
	Human FSTL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50-for this effect is typically 5-25 ng/mL in the presence of 7.5 ng/mL rhActivin A.
	The purity of Human FSTL3 is greater than 90% as determined by SEC-HPLC.

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