Name: Mouse GFRA3 Protein (GFR-MM1A3)
Brand: KactusBio
SKU: GFR-MM1A3-100UG
Price: 330.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse ARTN, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse GFRA3, His Tag with the EC50 of 54.7ng/ml determined by ELISA (QC Test).
Amino Acid Range: Asn29-Arg379

Catalog #GFR-MM1A3

Size

100UG

500UG

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Mouse GFRA3 Protein (GFR-MM1A3) Summary

Product Description	Recombinant Mouse GFRA3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asn29-Arg379.
Product Background	GDNF (glial-cell-line derived neurotrophic factor) is a potent neurotrophic factor for dopaminergic neurons. Neuropsychiatric diseases and their treatments are associated with alterations in the levels of both GDNF and its receptor family (GDNF family receptor alpha or GFRA). GFRA1, GFRA2 and GFRA3 are located in chromosomal regions with suggestive linkage to schizophrenia.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Mouse GFRA3 Protein (GFR-MM1A3) Specifications/Details

Protein	GFRA3
Synonyms	GDNFR-alpha-3; GFR-alpha-3; GFRA3; GFR alpha3; GPI-linked receptor
Accession	O35118
Species	Mouse
Biotinylated	no
Amino Acid Range	Asn29-Arg379
Molecular Weight	The protein has a predicted MW of 40.3 kDa. Due to glycosylation, the protein migrates to 47-52 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Mouse ARTN, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse GFRA3, His Tag with the EC50 of 54.7ng/ml determined by ELISA (QC Test).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Mouse ARTN, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse GFRA3, His Tag with the EC50 of 54.7ng/ml determined by ELISA (QC Test).
	Mouse GFRA3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Mouse GFRA3, His Tag on Anti-His-Biosensor can bind Mouse ARTN, hFc Tag with an affinity constant of 1.07 nM as determined in BLI assay .
	The purity of Mouse GFRA3 is greater than 95% as determined by SEC-HPLC.

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