• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-Arg-Pro-Lys-Pro-Val-Glu-NVAL-Trp-Arg-Lys(DNP)-NH2. The IC50 is < 1 nM.
  • Amino Acid Range: Gly36-Val433
Catalog #HAI-HM11A
Price
Regular price $34650
Regular price Sale price $34650
/
Size
Avantor VWR Fisher Scientific

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Human HAI-1 Protein (HAI-HM11A) Summary

Product Description

 Recombinant Human HAI-1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly36-Val433.

Product Background

 SPINT1, also known as HAI-1, is a Kunitz-type serine protease inhibitor that inhibits multiple proteases including hepatocyte growth factor (HGF) activator and matriptase. SPINT1 has been shown to modulate HGF/MET activation in certain cancer types.

Human HAI-1 Protein (HAI-HM11A) Specifications/Details

Protein
 HAI-1
Synonyms
 HAI 1; SPINT1; HAI1; HAI-1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly36-Val433
Molecular Weight
 The protein has a predicted MW of 45.50 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity
 Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-Arg-Pro-Lys-Pro-Val-Glu-NVAL-Trp-Arg-Lys(DNP)-NH2. The IC50 is < 1 nM.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human HAI-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human HAI-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HAI-1 is greater than 95% as determined by SEC-HPLC. The purity of Human HAI-1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...