- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: The affinity constant of 20.96 uM as determined in SPR assay (Biacore T200).
- Amino Acid Range: Leu22-Ser195
Human Interferon omega-1 Protein (IFN-HM101) Summary
Product Description |
Recombinant Human Interferon omega-1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Leu22-Ser195. |
Product Background |
Human Interferon omega-1, a type I IFN, also known as IFN-ω1 or IFNW1, it contains a potential N-glycosylation site like human IFN-β. IFN-ω1 binds to the interferon alpha/beta receptor but not to the interferon gamma receptor. All IFN-ω genes analysed to date are devoid of introns. IFN-ω1 has been shown to be well-tolerated in man and to induce reductions of hepatitis C virus RNA levels in a series of human clinical trials. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human Interferon omega-1 Protein (IFN-HM101) Specifications/Details
Protein |
Interferon omega-1 |
Synonyms |
Interferon alpha-II-1; IFNW1; IFN-ω1; IFN-omega-1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu22-Ser195 |
Molecular Weight |
The protein has a predicted MW of 21.25 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 20.96 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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