Regular price
$577.50
Order by 4pm ET for delivery on:
Biotinylated Human LDLR Protein (LDL-HM401B) Summary
Product Description |
Recombinant Biotinylated Human LDLR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala22-Arg788. |
Product Background |
The low density lipoprotein receptor (LDLR) is the founding member of the LDL R family of widely expressed cell surface scavenger receptors. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Biotinylated Human LDLR Protein (LDL-HM401B) Specifications/Details
Protein |
LDLR |
Synonyms |
LDLR; FHC; LDL R; LDL receptor; LDLCQ2; FH |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ala22-Arg788 |
Molecular Weight |
The protein has a predicted MW of 87.6 kDa, Due to glycosylation, the protein migrates to 120-140 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.23 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Biotinylated Human LDLR, His Tag immobilized on CM5 Chip can bind Human PCSK9, His Tag with an affinity constant of 0.23 nM as determined in SPR assay (Biacore T200). |
|
|
Biotinylated Human LDLR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Biotinylated Human LDLR is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
