Human LILRB2/CD85d/ILT4 Protein (LIL-HM2B2) Summary
Product Description |
Recombinant Human LILRB2/CD85d/ILT4 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Gln22-Arg457. |
Product Background |
The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC). LILRB2 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Human LILRB2/CD85d/ILT4 Protein (LIL-HM2B2) Specifications/Details
Protein |
LILRB2/CD85d/ILT4 |
Synonyms |
CD85d; ILT4; ILT-4; ILT4CD85d; LILRB2; LIR2; MIR10 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln22-Arg457 |
Molecular Weight |
The protein has a predicted MW of 74.1 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human LILRB2, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LILRB2 Antibody, hFc Tag with the EC50 of 35.4ng/ml determined by ELISA (QC Test). The affinity constant of 9.20nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-hFc (IgG1) |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM Tris, 100mM glycine, 100mM L-arginine, 150mM NaCl (pH 7.5) / PBS, 200mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human LILRB2, hFc tag captured on Protein A Chip can bind Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag (Cat. HLG-HM41CT) with an affinity constant of 9.20nM as determined in SPR assay (Biacore T200). |
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Human LILRB2, hFc Tag captured on Protein A chip, can bind Human ANGPTL2, His Tag (Cat. ANG-HM4L2) with an affinity constant of 0.14uM as determined in a SPR assay (Biacore T200). |
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Immobilized Human LILRB2, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LILRB2 Antibody, hFc Tag with the EC50 of 35.4ng/ml determined by ELISA (QC Test). |
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Immobilized Biotinylated Human ANGPTL7, His Avi Tag (Cat. ANG-HM407) at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LILRB2, hFc Tag with the EC50 of 3.09ug/ml determined by ELISA. |
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Human LILRB2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Human LILRB2, hFc Tag on ProA-Biosensor can bind Human ANGPTL4, His Tag (Cat. ANG-HM1L4) with an affinity constant of 72.10 nM as determined in BLI assay (Gator® Prime). |
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The purity of-Human LILRB2 is greater than 95% as determined by SEC-HPLC. |
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