• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human RGMa, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA.
  • Amino Acid Range: Ala34-Leu1105
Catalog #NEO-HM201
Price
Regular price $34650
Regular price Sale price $34650
/
Size
Avantor VWR Fisher Scientific

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Human Neogenin Protein (NEO-HM201) Summary

Product Description

 Recombinant Human Neogenin Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ala34-Leu1105.

Product Background

 Neogenin is a multifunctional transmembrane receptor belonging to the immunoglobulin superfamily. It displays identical secondary structure to deleted in colorectal cancer (DCC), a netrin receptor that is involved in axon guidance and cell survival.

Human Neogenin Protein (NEO-HM201) Specifications/Details

Protein
 Neogenin
Synonyms
 NEO1; IGDCC2; NGN; NEO; neogenin 1;
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala34-Leu1105
Molecular Weight
 The protein has a predicted MW of 144.37 kDa. Due to glycosylation, the protein migrates to 150-180 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human RGMa, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA.
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human RGMa, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA. Immobilized Human RGMa, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA.
Human Neogenin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Neogenin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Neogenin is greater than 95% as determined by SEC-HPLC. The purity of Human Neogenin is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...