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Biotinylated Human Notch 2 Protein (NOT-HM402B) Summary
Product Description |
Recombinant Biotinylated Human Notch 2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu26-Gln530. |
Product Background |
Human Notch-2 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes. Although Notch proteins are structurally and functionally similar, deletion of either Notch-1 or Notch-2 is lethal, showing that not all functions overlap.Notch-2 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Biotinylated Human Notch 2 Protein (NOT-HM402B) Specifications/Details
Protein |
Notch 2 |
Synonyms |
AGS2; Notch-2; NOTCH2; N2ECD; HJCYS; N2 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Leu26-Gln530 |
Molecular Weight |
The protein has a predicted MW of 57 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Biotinylated Human Notch 2 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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The purity of Biotinylated Human Notch 2 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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