Canine PDGF-AA Protein (PDF-DY10A) Summary
Product Description |
Recombinant Canine PDGF-AA Protein is expressed from Yeast with His tag at the N-terminus.It contains Ser87-Arg196. |
Product Background |
Platelet-derived growth factors (PDGFs) may play an important role in the development of atherosclerosis acting as chemoattractants and mitogens for vascular smooth muscle cells and macrophages. PDGFA, the most known member of PDGF family, plays a crucial role in occurrence and progression of different tumors. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Canine PDGF-AA Protein (PDF-DY10A) Specifications/Details
Protein |
PDGF-AA |
Synonyms |
PDGF-AA; PDGF-A; PDGF1; PDGF A; PDGFAA; PDGF AA |
Accession |
|
Species |
Canine |
Biotinylated |
no |
Amino Acid Range |
Ser87-Arg196 |
Molecular Weight |
The protein has a predicted MW of 13.62 kDa. Due to glycosylation, the protein migrates to 15-20 kDa and 35-45 kDa based on Bis-Tris PAGE result. |
Product Tag |
N-His |
Expression System |
Yeast |
Purity |
> 90% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 4mM HCL. Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in 4mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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