Canine PDGF-AA Protein (PDF-DY10A)
Canine PDGF-AA Protein (PDF-DY10A)
  • Express System: Yeast
  • Product Tag: N-His
  • Purity: > 90% as determined by Bis-Tris PAGE
  • Amino Acid Range: Ser87-Arg196
Catalog #PDF-DY10A
Regular price $379.50
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Canine PDGF-AA Protein (PDF-DY10A) Summary

Product Description

Recombinant Canine PDGF-AA Protein is expressed from Yeast with His tag at the N-terminus.It contains Ser87-Arg196.

Product Background

Platelet-derived growth factors (PDGFs) may play an important role in the development of atherosclerosis acting as chemoattractants and mitogens for vascular smooth muscle cells and macrophages. PDGFA, the most known member of PDGF family, plays a crucial role in occurrence and progression of different tumors.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Canine PDGF-AA Protein (PDF-DY10A) Specifications/Details

Protein
PDGF-AA
Synonyms
PDGF-AA; PDGF-A; PDGF1; PDGF A; PDGFAA; PDGF AA
Accession
Species
Canine
Biotinylated
no
Amino Acid Range
Ser87-Arg196
Molecular Weight
The protein has a predicted MW of 13.62 kDa. Due to glycosylation, the protein migrates to 15-20 kDa and 35-45 kDa based on Bis-Tris PAGE result.
Product Tag
N-His
Expression System
Yeast
Purity
> 90% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 4mM HCL. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in 4mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Canine PDGF-AA on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. Canine PDGF-AA on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50-for this effect is 5 - 15 ng/mL. Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50-for this effect is 5 - 15 ng/mL.
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