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Mouse SP17 Protein (SP7-ME117) Summary
Product Description |
Recombinant Mouse SP17 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Met1-Asn149. |
Product Background |
Sperm protein 17 (Sp17) is a highly conserved mammalian protein characterized in rabbit, mouse, monkey, baboon, macaque, human testis and spermatozoa. mRNA encoding Sp17 has been detected in a range of murine and human somatic tissues. It was also recognized in two myeloma cell lines and in neoplastic cells from patients with multiple myeloma and ovarian carcinoma. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse SP17 Protein (SP7-ME117) Specifications/Details
Protein |
SP17 |
Synonyms |
CT22; Sp17-1; Sperm protein 17; SPA17; |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Met1-Asn149 |
Molecular Weight |
The protein has a predicted MW of 18.39 kDa. The protein migrates to 20-25 kDa based on Bis-Tris PAGE result. |
Product Tag |
N-His |
Fluorophore |
Unconjugated |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Mouse SP17 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Mouse SP17 is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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