Human TFPI-2 Protein (TFP-HM102)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, the IC50 value is < 5 nM.
Amino Acid Range: Asp23-Lys213.

Catalog #TFP-HM102

Price

$313⁵⁰

Size

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Human TFPI-2 Protein (TFP-HM102) Summary

Product Description

Recombinant Human TFPI-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp23-Lys213.

Product Background

Tissue factor pathway inhibitor-2 (TFPI-2) has previously been characterized as an endogenous anticoagulant. TFPI-2 is expressed in the vast majority of cells, mainly secreted into the extracellular matrix. Moreover, in sputum from cystic fibrosis patients TFPI-2 C-terminal fragments are generated and found associated with immunoglobulins. Together our data describe a previously unknown host defense mechanism and therapeutic importance of TFPI-2 against invading Gram-negative bacterial pathogens.

Human TFPI-2 Protein (TFP-HM102) Specifications/Details

Protein	TFPI-2
Synonyms	TFPI-2; PP5; TFPI2; REF1; TFPI-2REF1;TFPI2REF1
Accession	NP_006519.1
Species	Human
Biotinylated	no
Amino Acid Range	Asp23-Lys213.
Molecular Weight	The protein has a predicted MW of 22.9 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result.
Activity	Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, the IC50 value is < 5 nM.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.