Human Betacellulin-Protein (BTC-HE001) Summary
Product Description |
Recombinant Human Betacellulin-Protein is expressed from E.coli without tag.It contains Asp32-Gln118. |
Product Background |
Betacellulin (BTC) belongs to the epidermal growth factor (EGF) family of peptide ligands that are characterised by a six-cysteine consensus motif that forms three intra-molecular disulfide bonds crucial for binding the ErbB receptor family. |
Product Category |
Recombinant Protein / Cell therapy / Cell culture / Cytokines |
Human Betacellulin-Protein (BTC-HE001) Specifications/Details
Protein |
Betacellulin |
Synonyms |
Probetacellulin; BTC |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp32-Gln118 |
Molecular Weight |
The protein has a predicted MW of 9.76 kDa. The protein migrates to 14-18 kDa based on Bis-Tris PAGE result. |
Product Tag |
No Tag |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human Betacellulin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50-for this effect is 0.10-1.50 ng/ml (QC Test). |
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The purity of Human Betacellulin is greater than 95% as determined by SEC-HPLC. |
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