Mouse CD4/LEU3 Protein (CD4-MM101) Summary
Product Description |
Recombinant Mouse CD4/LEU3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys27-Thr394. |
Product Background |
CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets |
Mouse CD4/LEU3 Protein (CD4-MM101) Specifications/Details
Protein |
CD4/LEU3 |
Synonyms |
CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Lys27-Thr394 |
Molecular Weight |
The protein has a predicted MW of 42.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Mouse CD4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Mouse CD4 is greater than 95% as determined by SEC-HPLC. |
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