Name: Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein (MHC-HM445B)
Brand: KactusBio
SKU: MHC-HM445B-100UG
Price: 341.0 USD
Availability: InStock

HPLC chromatogram displaying a dominant peak at 9.645 minutes, confirming high purity of the recombinant protein.

Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein (MHC-HM445B)

Immobilized Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test).

Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KVLEYVIKV peptide

Catalog #MHC-HM445B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein (MHC-HM445B) Summary

Product Description	Recombinant Biotinylated Human HLA-A02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A02:01), Ile21-Met119(B2M) and KVLEYVIKV peptide.
Product Background	MAGE-A1 belongs to the chromosome X-clustered genes of cancer-testis antigen family and is normally expressed in the human germ line but is also overexpressed in various tumors.
Product Category	Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Specifications

Protein	HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV)
Synonyms	MAGE-A1; MAGE A1; CT1.1; MAGE-1 antigen; MAGE-1; MZ2-E; MAGE1; MAGE1A
MHC Form	Monomer
Allele	HLA-A*02:01
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&KVLEYVIKV
Species	Human
Biotinylated	yes
Amino Acid Range	Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KVLEYVIKV peptide
Molecular Weight	The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 53-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test).
	Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer is greater than 95% as determined by SEC-HPLC.