Name: Human Beta-NGF Protein (NGF-HM00B)
Brand: KactusBio
SKU: NGF-HM00B-100UG
Price: 247.5 USD
Availability: InStock

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human Beta-NGF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TrkA, His Tag with the EC50 of 0.25ug/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Ser122-Ala241

Catalog #NGF-HM00B

Size

100UG

500UG

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Human Beta-NGF Protein (NGF-HM00B) Summary

Product Description	Recombinant Human Beta-NGF Protein is expressed from HEK293 without tag.It contains Ser122-Ala241.
Product Background	Nerve growth factor-beta (β-NGF) is a polypeptide growth factor that regulates survival, growth, and differentiation of specific peripheral and central neurons via its high-affinity receptor, tyrosine kinase receptor A (TrKA), and low-affinity receptor, p75 neurotrophin receptor (p75NTR).
Product Category	Recombinant Protein / Cell therapy / Cell culture / Cytokines

Human Beta-NGF Protein (NGF-HM00B) Specifications/Details

Protein	Beta-NGF
Synonyms	NGF; NGFB; Beta NGF; HSAN5; β-NGF
Accession	P01138
Species	Human
Biotinylated	no
Amino Acid Range	Ser122-Ala241
Molecular Weight	The protein has a predicted MW of 13.49 kDa. Due to glycosylation, the protein migrates to 14-18 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Beta-NGF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TrkA, His Tag with the EC50 of 0.25ug/ml determined by ELISA. See testing image for detail.
Product Tag	No Tag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 20mM NaAc, 150mM Nacl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in 20mM NaAc, 150mM Nacl (pH 5.5). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human Beta-NGF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TrkA, His Tag with the EC50 of 0.25ug/ml determined by ELISA.
	Human Beta-NGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2 - 2 ng/ml.