Name: Human TPO/Thrombopoietin Protein (TPO-HM102)
Brand: KactusBio
SKU: TPO-HM102-100UG
Price: 297.0 USD
Availability: InStock

Human TPO/Thrombopoietin Protein (TPO-HM102)

Human c-MPL, His Tag immobilized on CM5 Chip can bind Human Thrombopoietin, His Tag with an affinity constant of 25.54 nM as determined in SPR assay (Biacore T200).

Human TPO on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50-for this effect is typically 0.5-5 ng/ml (QC Test).

The purity of Human TPO is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 25.54 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ser22-Gly353

Catalog #TPO-HM102

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human TPO/Thrombopoietin Protein (TPO-HM102) Summary

Product Description	Recombinant Human TPO/Thrombopoietin Protein is expressed from HEK293 with His tag at the N-terminus.It contains Ser22-Gly353.
Product Background	Thrombopoietin (TPO), the c-Mpl ligand, is produced constitutively in liver and other organs, circulates in the bloodstream, and is delivered to bone marrow, where it stimulates the early development of multiple hematopoietic lineages and megakaryocytopoiesis. The concentration of TPO in blood is regulated by c-Mpl mass on platelets and megakaryocytes.
Product Category	Recombinant Protein / Cell therapy / Cell culture / Cytokines

Human TPO/Thrombopoietin Protein (TPO-HM102) Specifications/Details

Protein	TPO/Thrombopoietin
Synonyms	Thrombopoietin; TPO; MGDF; THPO; ML; C-mpl ligand
Accession	NP_000451.1
Species	Human
Biotinylated	no
Amino Acid Range	Ser22-Gly353
Molecular Weight	The protein has a predicted MW of 36.56 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 25.54 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human c-MPL, His Tag immobilized on CM5 Chip can bind Human Thrombopoietin, His Tag with an affinity constant of 25.54 nM as determined in SPR assay (Biacore T200).
	Human TPO on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50-for this effect is typically 0.5-5 ng/ml (QC Test).
	The purity of Human TPO is greater than 95% as determined by SEC-HPLC.