Name: Biotinylated Human VEGF121 Protein (VEG-HM421B)
Brand: KactusBio
SKU: VEG-HM421B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human VEGF121 Protein (VEG-HM421B)

Immobilized Human VEGF R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF121, His Tag with the EC50 of 40.2ng/ml determined by ELISA.

Biotinylated Human VEGF121 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human VEGF R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF121, His Tag with the EC50 of 40.2ng/ml determined by ELISA.
Amino Acid Range: Ala27-Arg147

Catalog #VEG-HM421B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human VEGF121 Protein (VEG-HM421B) Summary

Product Description	Recombinant Biotinylated Human VEGF121 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Arg147.
Product Background	Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human VEGF121 Protein (VEG-HM421B) Specifications/Details

Protein	VEGF121
Synonyms	VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609
Accession	P15692-9
Species	Human
Biotinylated	yes
Amino Acid Range	Ala27-Arg147
Molecular Weight	The protein has a predicted MW of 17 kDa. Due to glycosylation, the protein migrates to 18 kDa and 22-25 kDa under reduced (R) condition, 30 kDa and 32-40 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Activity	Immobilized Human VEGF R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF121, His Tag with the EC50 of 40.2ng/ml determined by ELISA.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human VEGF R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF121, His Tag with the EC50 of 40.2ng/ml determined by ELISA.
	Biotinylated Human VEGF121 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.