Name: Non-biotinylated Human TPBG/5T4 Protein (5T4-HM401)
Brand: KactusBio
SKU: 5T4-HM401-100UG
Price: 346.5 USD
Availability: InStock

ELISA binding curve showing dose-dependent response of anti-TPBG antibody to recombinant human TPBG protein with His tag

Immobilized Human TPBG, His Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA.

Human TPBG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human TPBG is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human TPBG, His Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Ser32-Ser355

Catalog #5T4-HM401

Size

100UG

500UG

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Non-biotinylated Human TPBG/5T4 Protein (5T4-HM401) Summary

Product Description	Recombinant Human TPBG/5T4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus; It contains Ser32-Ser355.
Product Background	The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Non-biotinylated Human TPBG/5T4 Protein (5T4-HM401) Specifications/Details

Protein	TPBG/5T4
Synonyms	M6P1; 5T4AG; WAIF1; TPBG; 5T4
Accession	Q13641-1
Species	Human
Biotinylated	no
Amino Acid Range	Ser32-Ser355
Molecular Weight	The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human TPBG, His Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human TPBG, His Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA.
	Human TPBG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human TPBG is greater than 95% as determined by SEC-HPLC.