FITC-Labeled Human TPBG/5T4 Protein (5T4-HM401F)
FITC-Labeled Human TPBG/5T4 Protein (5T4-HM401F)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized FITC-Labeled Human TPBG, His Tag at 0.5ug/ml(100ul/well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Ser32-Ser355
Catalog #5T4-HM401F
Regular price $990.00
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FITC-Labeled Human TPBG/5T4 Protein (5T4-HM401F) Summary

Product Description

Recombinant FITC-Labeled Human TPBG/5T4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser32-Ser355.

Product Background

The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets

FITC-Labeled Human TPBG/5T4 Protein (5T4-HM401F) Specifications/Details

Protein
TPBG/5T4
Synonyms
M6P1; 5T4AG; WAIF1; TPBG; 5T4
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ser32-Ser355
Molecular Weight
The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized FITC-Labeled Human TPBG, His Tag at 0.5ug/ml(100ul/well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA (QC Test).
Product Tag
C-His-Avi

Fluorophore

FITC
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 6 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized FITC-Labeled Human TPBG, His Tag at 0.5ug/ml(100ul/well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA (QC Test). Immobilized FITC-Labeled Human TPBG, His Tag at 0.5ug/ml(100ul/well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA (QC Test).
FITC-Labeled Human TPBG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. FITC-Labeled Human TPBG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of FITC-Labeled Human TPBG is greater than 95% as determined by SEC-HPLC. The purity of FITC-Labeled Human TPBG is greater than 95% as determined by SEC-HPLC.
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